Isolation and Partial Analysis of a Polysaccharide-protein Complex from Lactarius Deliciosus
نویسندگان
چکیده
A crude polysaccharide-protein complex from Lactarius deliciosus fruit bodies (LDP) was extracted with hot water, precipitated, and washed with 95% ethanol. After protein removal according to the Sevag method, LDP was dialyzed against running tap water and distilled water, and further purified by Sephadex G-100 column chromatography. Gas chromatography showed that LDP is composed of glucose, galactose, mannose, and arabinose in a molar ratio of 100:14.9:6.23:1.97. According to Gel Permeation Chromatography-HPLC results, the molecular weight is 642kDa. An IR spectrum revealed coinciding polysaccharide and peptide absorption peaks, while a 1 H-NMR spectrum indicated the main chain has a ß-pyranglycoside linkage. Amino acid analysis detected the presence of 17 kinds of amino acids.
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